Egg-grown and tissue-culture-grown variants of influenza A (H3N2) virus with special attention to their use as antigens in seroepidemiology
Identifieur interne : 002304 ( Main/Exploration ); précédent : 002303; suivant : 002305Egg-grown and tissue-culture-grown variants of influenza A (H3N2) virus with special attention to their use as antigens in seroepidemiology
Auteurs : R. Pyh L [Finlande] ; L. Pyh L [Finlande] ; M. Valle [Finlande] ; K. Aho [Finlande]Source :
- Epidemiology and Infection [ 0950-2688 ] ; 1987-12.
Abstract
A field strain of influenza A (H3N2) virus isolated in embryonated eggs during the 1984–5 influenza outbreak (A/Finland/13/85E) was compared in an antigenic analysis with virus from the same clinical specimen isolated in MDCK cell cultures (A/Finland/13/85M). The M-virus appeared to be more sensitive to haemagglutination-inhibiting antibodies against heterologous viruses than did the Evirus. The results of propagation and plaque purification experiments support the hypothesis that a single clinical specimen may consist of distinct antigenic variant subpopulations promoted selectively by the host during isolation procedures. Receptor-binding properties are discussed as a possible explanation for this selectivity. A set of 471 paired sera consisting of pre-epidemic and post-epidemic specimens taken from the same subjects in 1984–5 was studied for haemagglutinationinhibiting antibodies to six influenza A (H3N2) virus strains, including the E-virus and the M-virus from A/Finland/13/85. Of the antigens used, the M-virus detected significant antibody increases more frequently than did the E-virus (10·0 v. 5·9%). The superiority of the M-virus may rest primarily in its ability to pick out anamnestic antibody responses. Irrespective of this cross-reactivity, preepidemic antibody to the M-virus was fairly well associated with protection. In the set of sera (230 specimens) collected in summer 1985 to represent different age groups, the antibody status against the M-virus was significantly better than the status against the E-virus. The results suggest that, at least in some instances, antibody to MDCK-grown virus is a more accurate indicator of the immune status of a community than antibodies to egg-grown virus variants.
Url:
DOI: 10.1017/S0950268800066607
Affiliations:
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Pyh L</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki</wicri:regionArea><wicri:noRegion>SF-00280 Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Pyh L, L" sort="Pyh L, L" uniqKey="Pyh L L" first="L." last="Pyh L">L. Pyh L</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki</wicri:regionArea><wicri:noRegion>SF-00280 Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Valle, M" sort="Valle, M" uniqKey="Valle M" first="M." last="Valle">M. 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Infect.</title><idno type="ISSN">0950-2688</idno><idno type="eISSN">1469-4409</idno><imprint><publisher>Cambridge University Press</publisher><pubPlace>Cambridge, UK</pubPlace><date type="published" when="1987-12">1987-12</date><biblScope unit="volume">99</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="745">745</biblScope><biblScope unit="page" to="753">753</biblScope></imprint><idno type="ISSN">0950-2688</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0950-2688</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">A field strain of influenza A (H3N2) virus isolated in embryonated eggs during the 1984–5 influenza outbreak (A/Finland/13/85E) was compared in an antigenic analysis with virus from the same clinical specimen isolated in MDCK cell cultures (A/Finland/13/85M). The M-virus appeared to be more sensitive to haemagglutination-inhibiting antibodies against heterologous viruses than did the Evirus. The results of propagation and plaque purification experiments support the hypothesis that a single clinical specimen may consist of distinct antigenic variant subpopulations promoted selectively by the host during isolation procedures. Receptor-binding properties are discussed as a possible explanation for this selectivity. A set of 471 paired sera consisting of pre-epidemic and post-epidemic specimens taken from the same subjects in 1984–5 was studied for haemagglutinationinhibiting antibodies to six influenza A (H3N2) virus strains, including the E-virus and the M-virus from A/Finland/13/85. Of the antigens used, the M-virus detected significant antibody increases more frequently than did the E-virus (10·0 v. 5·9%). The superiority of the M-virus may rest primarily in its ability to pick out anamnestic antibody responses. Irrespective of this cross-reactivity, preepidemic antibody to the M-virus was fairly well associated with protection. In the set of sera (230 specimens) collected in summer 1985 to represent different age groups, the antibody status against the M-virus was significantly better than the status against the E-virus. The results suggest that, at least in some instances, antibody to MDCK-grown virus is a more accurate indicator of the immune status of a community than antibodies to egg-grown virus variants.</div></front></TEI><affiliations><list><country><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Pyh L, R" sort="Pyh L, R" uniqKey="Pyh L R" first="R." last="Pyh L">R. Pyh L</name></noRegion><name sortKey="Aho, K" sort="Aho, K" uniqKey="Aho K" first="K." last="Aho">K. Aho</name><name sortKey="Pyh L, L" sort="Pyh L, L" uniqKey="Pyh L L" first="L." last="Pyh L">L. Pyh L</name><name sortKey="Valle, M" sort="Valle, M" uniqKey="Valle M" first="M." last="Valle">M. Valle</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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